REV-ERB agonist raises a handful of sensible questions. This page answers them in order, starting with the fundamentals and moving to applications.
This page was last updated on 2025-09-25 and is reviewed periodically as new material appears.
SR9009 is often grouped with compounds studied for circadian and metabolic regulation rather than with classical anabolic steroids. Its interactions with nuclear receptors differ from those of androgen receptor ligands, and its proposed mechanisms involve transcriptional control rather than direct hormone signaling. Some sources classify it as a metabolic modulator because of observed effects on energy utilization. The distinction matters for regulation and for interpreting research results across different compound classes.
SR9009 is a synthetic small molecule developed as a REV-ERB agonist. It binds to REV-ERBα and REV-ERBβ, nuclear receptors that help regulate circadian rhythms and metabolic gene expression. In cell and animal studies, the compound alters lipid and glucose handling and influences skeletal muscle oxidative capacity. Its exact effects in humans remain largely uncharacterized because controlled clinical trials have not been reported. The molecule is frequently described in preclinical literature as a metabolic modulator.
Regulatory and sporting contexts treat SR9009 as a prohibited substance in many elite competitions. Its presence on banned lists reflects concerns about performance enhancement and unknown long-term safety. Analytical chemists have developed methods to detect the parent compound and its metabolites in urine and blood. Literature discussions distinguish between in vitro potency, animal pharmacology, and anecdotal human reports. The latter are difficult to verify because products sold online may lack purity or contain different compounds.
SR9009 is a synthetic small molecule studied as an agonist of the nuclear receptors REV-ERBα and REV-ERBβ. These receptors help regulate circadian rhythms, lipid metabolism, and inflammatory gene expression. In cell and animal experiments, SR9009 alters transcription of clock-controlled genes and metabolic pathways. The compound is not a hormone and does not resemble classical steroid structures. Its activity depends on binding to the ligand-binding domain of REV-ERB, where it can modify corepressor recruitment.
| Property | Value | Notes |
|---|---|---|
| Molecular formula | C20H24ClN3O4S | Reported for the free base |
| Molecular weight | 437.9 g/mol | Calculated from the formula |
| Primary target | REV-ERBα and REV-ERBβ | Nuclear receptors involved in circadian regulation |
| Solubility | Soluble in DMSO and ethanol | Poorly soluble in water |
| Typical storage | -20 °C, desiccated | Protect from light and moisture |
Regulatory treatment of SR9009 varies by country and context. It is not approved as a therapeutic drug by agencies such as the United States Food and Drug Administration or the European Medicines Agency. Sports authorities list it as a prohibited substance; the World Anti-Doping Agency classifies it among hormone and metabolic modulators. Legal status for personal possession or sale differs across jurisdictions, and some countries may restrict it under analog or research chemical laws. Buyers who seek verified material often rely on independent laboratory testing because online product labels may not match contents.
Laboratory samples of SR9009 are typically handled as research chemicals rather than pharmaceuticals. Suppliers usually state that the material is for research use only and not for human or veterinary administration. Storage recommendations generally call for a freezer at approximately −20 °C, protection from light, and a desiccated environment. The solid is often described as a white to off-white powder. Solubility is commonly reported in organic solvents such as dimethyl sulfoxide and ethanol, with low solubility in water.
Analytical identification and purity assessment often use high-performance liquid chromatography with ultraviolet detection or mass spectrometry. Liquid chromatography–tandem mass spectrometry is used to detect and quantify SR9009 in biological matrices, including urine and blood, for anti-doping or pharmacokinetic studies. Nuclear magnetic resonance spectroscopy can confirm molecular structure. Stability depends on form and storage: the solid is generally more stable than solutions, and repeated freeze–thaw cycles may degrade samples. Purity is typically reported as a percentage from a certificate of analysis.
Physicochemical behavior influences handling. SR9009 is described as a solid with limited aqueous solubility, so organic solvents such as dimethyl sulfoxide or ethanol are common in research stock solutions. Aqueous dilution can produce precipitates if the organic content is too low. Light, heat, and repeated freeze-thaw cycles may affect stability. Storage recommendations usually specify a desiccated freezer environment protected from light, but exact stability data depend on the formulation and matrix.
Detection in biological samples can be complicated by rapid metabolism and low circulating concentrations. Some studies report phase I and phase II metabolites, and analytical methods may need to target those species in addition to the parent compound. Immunoassays are not broadly available, so mass spectrometry remains the main confirmatory approach. For anti-doping testing, laboratories look for SR9009 and its metabolites using validated LC-MS methods. Open questions include how long metabolites remain detectable and how different routes of administration alter detection windows.
In laboratory settings, SR9009 is typically characterized by liquid chromatography–mass spectrometry (LC-MS) or high-performance liquid chromatography with ultraviolet detection (HPLC-UV). These methods can confirm identity and estimate purity, but they require reference standards for accurate quantification. Because SR9009 is not a licensed pharmaceutical, no harmonized pharmacopeial monograph exists. Laboratories often validate in-house methods for matrices such as plasma, urine, or cell culture media. Sample preparation may involve protein precipitation or liquid-liquid extraction before analysis.
Analytical identification of SR9009 typically relies on liquid chromatography coupled with tandem mass spectrometry. In biological samples, researchers first separate the compound from matrix components using protein precipitation, liquid-liquid extraction, or solid-phase extraction. High-performance liquid chromatography with ultraviolet detection and nuclear magnetic resonance spectroscopy can support structural confirmation of reference materials. Because SR9009 is a small, relatively lipophilic molecule, reverse-phase columns and acidic mobile phases are common. Laboratories often include isotope-labeled internal standards to improve quantification and to correct for ion suppression.
Stability depends on physical form, temperature, light exposure, and solvent. Solid SR9009 is generally stored cold and dry, with protection from light to limit degradation. Dimethyl sulfoxide stocks are common for laboratory work, but repeated freeze-thaw cycles can reduce compound integrity. Aqueous solutions may be less stable than organic stocks, and the ethyl ester in the structure can be susceptible to hydrolysis under certain conditions. Researchers typically validate storage conditions and recheck purity before quantitative experiments, especially when using archived material.
SR9009 is a synthetic small molecule developed as an agonist of the nuclear receptors REV-ERBα (NR1D1) and REV-ERBβ (NR1D2). These receptors help regulate circadian rhythms and metabolic gene expression. In cell and animal studies, SR9009 alters transcription of genes involved in lipid metabolism, inflammation, and mitochondrial function. It is not an approved medicine, and its pharmacological profile in humans remains largely uncharacterized. The compound is frequently discussed in the context of circadian biology and metabolic research rather than clinical use.
Preclinical reports have linked SR9009 to improved endurance and altered energy expenditure in rodents. Such findings have prompted interest in whether REV-ERB activation can influence skeletal muscle metabolism. However, the reported effects depend on dose, route, and experimental model, and replication across laboratories is limited. Human trials have not established comparable outcomes, so claims about exercise performance remain speculative. The absence of controlled human data is a central limitation in interpreting these observations.
The mechanism of action involves binding to REV-ERB receptors and recruiting corepressor complexes, which represses target gene transcription. This contrasts with many nuclear receptor agonists that activate transcription. Downstream effects may include changes in autophagy, mitochondrial biogenesis, and lipid handling, but the precise pathways remain an active area of study. Whether these molecular events translate into meaningful physiological effects in humans is unresolved. Most evidence comes from cultured cells and rodent models rather than human participants.
=== Purification of heterotetramers === Ion-exchange chromatography is useful for isolating specific heterotetrameric protein assemblies, allowing purification of specific complexes according to both the number and the position of charged peptide tags. Nickel affinity chromatography may also be employed for heterotetramer purification.
== Dietary requirements == The Food and Nutrition Board (FNB) of the U.S. Institute of Medicine set Recommended Dietary Allowances (RDAs) for essential amino acids in 2002. For leucine, for adults 19 years and older, 42 mg/kg body weight/day; for isoleucine 19 mg/kg body weight/day; for valine 24 mg/kg body weight/day. For a 70 kg (154 lb) person this equates to 2.9, 1.3 and 1.7 g/day. Diets that meet or exceed the RDA for total protein (0.8 g/kg/day; 56 grams for a 70 kg person), meet or exceed the RDAs for branched-chain amino acids.
== June 2, 1915 (Wednesday) == Second Battle of Artois — The French bombarded German defenses around Neuville-Saint-Vaast, France for three days before launching an attack. Shabin-Karahisar uprising — An Armenian militia of 250 began a month-long resistance against the Ottoman Empire in response to the Armenian genocide using a fort in the Giresun Province of Turkey. Ceylon Governor Robert Chalmers declared martial law in the colony (now Sri Lanka) after violent unrest between Muslim and Buddhist broke out in Kandy and spread to other towns and cities. The Imperial German Army established the 83rd and 84th Infantry Divisions. Born: Walter Tetley, American voice actor, best known as the voice of "Sherman" in the TV cartoon Mr. Peabody; as Walter Campbell Tetzlaff, in New York City, United States (d. 1975) Lester del Rey, American science fiction writer and publisher, editor with wife Judy-Lynn del Rey of Del Rey Books; as Leonard Knapp, in Saratoga Township, Winona County, Minnesota, United States (d. 1993) Li Lili, Chinese actress, known for film roles in Little Toys and The Big Road; as Qian Zhenzhen, in Beijing, Republic of China (present-day China) (d. 2005) Died: George Randell, 84, Australian politician, Member of the Western Australian Legislative Council from 1873 to 1875 and 1880 to 1890 (b. 1830) Dave Orr, 55, American baseball player, first baseman for various teams including the New York Metropolitans and the Brooklyn Bridegrooms from 1883 to 1890 (b. 1859)
Static self-assembly is significantly slower compared to dynamic self-assembly as it depends on the random chemical interactions between particles. Self assembly can be directed in two ways. The first is by manipulating the intrinsic properties which includes changing the directionality of interactions or changing particle shapes. The second is through external manipulation by applying and combining the effects of several kinds of fields to manipulate the building blocks into doing what is intended. To do so correctly, a high level of direction and control is required and developing a simple, efficient method to organize molecules and molecular clusters into precise, predetermined structures is crucial.
=== July === July 1 Trade Representative Jamieson Greer announces that the US will not renew the USMCA free trade agreement signed during Trump's first administration. Trump takes his first flight in the new Air Force One, which was gifted by Qatar. Three American soldiers are injured and one soldier is missing after their MH-60S Seahawk helicopter went down in the Arabian Sea. July 3 2026 North American heat wave: Heat waves are reported across the eastern and central United States, with Independence Day celebrations cancelled or postponed including the Great American State Fair on the National Mall. Around 23 U.S. states are placed under heat warnings by the National Weather Service. A man dies after setting himself on fire outside the UN in New York, with activists and reports identifying him as a pro-Tibet advocate. Democrat Harry Benton resigns from the Illinois House of Representatives after allegations of misconduct.
Sources: en.wikipedia.org
There are 37 known isotopes of francium ranging in atomic mass from 197 to 233. Francium has seven metastable nuclear isomers. Francium-223 and francium-221 are the only isotopes that occur in nature, with the former being far more common. Francium-223 is the most stable isotope, with a half-life of 21.8 minutes, and it is highly unlikely that an isotope of francium with a longer half-life will ever be discovered or synthesized. Francium-223 is a fifth product of the uranium-235 decay series as a daughter isotope of actinium-227; thorium-227 is the more common daughter. Francium-223 then decays into radium-223 by beta decay (1.149 MeV decay energy), with a minor (0.006%) alpha decay path to astatine-219 (5.4 MeV decay energy). Francium-221 has a half-life of 4.8 minutes. It is the ninth product of the neptunium decay series as a daughter isotope of actinium-225. Francium-221 then decays into astatine-217 by alpha decay (6.457 MeV decay energy). Although all primordial 237Np is extinct, the neptunium decay series continues to exist naturally in tiny traces due to (n,2n) knockout reactions in natural 238U. Francium-222, with a half-life of 14 minutes, may be produced as a result of the beta decay of natural radon-222; this process has nonetheless not yet been observed, and it is unknown whether this process is energetically possible. The least stable ground state isotope is francium-215, with a half-life of 90 ns: it undergoes a 9.54 MeV alpha decay to astatine-211.
Additionally, a 2024 meta-analytic systematic review reported moderate improvements in quality of life when amphetamine treatment is used for ADHD. One review highlighted a nine-month randomized controlled trial of amphetamine treatment for ADHD in children that found an average increase of 4.5 IQ points, continued increases in attention, and continued decreases in disruptive behaviors and hyperactivity. Another review indicated that, based upon the longest follow-up studies conducted to date, lifetime stimulant therapy that begins during childhood is continuously effective for controlling ADHD symptoms and reduces the risk of developing a substance use disorder as an adult. Models of ADHD suggest that it is associated with functional impairments in some of the brain's neurotransmitter systems; these functional impairments involve impaired dopamine neurotransmission in the mesocorticolimbic projection and norepinephrine neurotransmission in the noradrenergic projections from the locus coeruleus to the prefrontal cortex. Stimulants like methylphenidate and amphetamine are effective in treating ADHD because they increase neurotransmitter activity in these systems. Approximately 80% of those who use these stimulants see improvements in ADHD symptoms. Children with ADHD who use stimulant medications generally have better relationships with peers and family members, perform better in school, are less distractible and impulsive, and have longer attention spans.
The term "Holstein" derives from Old Saxon Holseta Land (Holz means wood in modern Standardized German; holt is a now-archaic English word for woods.) Originally, the term referred to the central of the three Saxon tribes north of the River Elbe: Tedmarsgoi (Dithmarschen), Holstein, and Sturmarii (Stormarn). The area inhabited by the tribe of the Holsts lay between the Stör River and Hamburg; after Christianization, their main church was in Schenefeld. Saxon Holstein became a part of the Holy Roman Empire after Charlemagne's Saxon campaigns in the late eighth century. Beginning in 811, the northern border of Holstein (and thus of the Empire) was the River Eider. The term "Schleswig" originally referred to the city of Schleswig. The word Schleswig is a German transliteration of the Danish word Slesvig, which consists of two words: Schlei and vig. The Schlei refers to the river at which the city lies, and vig means "inlet" or bay. Schleswig, therefore, means (in Danish): "The bay at the river Schlei". The Schlei is known as Slien in Danish and is believed to have been used only for the inner Slien (the Great and Little Bay near the city of Schleswig). The word is thought to be related to Slæ, which means reeds and aquatic plants found in this area. The Duchy of Schleswig, or Southern Jutland, was originally an integral part of Denmark, but in medieval times was established as a fief under the control of the Kingdom of Denmark, having the same relationship with the Danish Crown as, for example, Brandenburg or Bavaria had with the Holy Roman Emperor.
== Characters == The story, as a whole, does not feature a single character who constantly plays the leading role. Each major narrative arc (e.g., Dance of..., The Archer of...) features its corresponding Agent and Guard or Archer and Custodian.
Sponges have medicinal potential due to the presence in sponges themselves or their microbial symbionts of chemicals that may be used to control viruses, bacteria, tumors and fungi. Lacking any protective shell or means of escape, sponges have evolved to synthesize a variety of unusual compounds. One such class is the oxidized fatty acid derivatives called oxylipins. Members of this family have been found to have anti-cancer, anti-bacterial and anti-fungal properties. One example isolated from the Okinawan Plakortis sponges, plakoridine A, has shown potential as a cytotoxin to murine lymphoma cells.
Sources: en.wikipedia.org
It is a synthetic REV-ERB agonist used mainly in preclinical research. It is not an approved medicine for human use.
It binds to REV-ERBα and REV-ERBβ, nuclear receptors involved in circadian and metabolic gene regulation. This can alter transcription of genes related to lipid and glucose metabolism in experimental models.
No controlled human trials have established that effect. Endurance findings come primarily from rodent studies, and human responses remain unknown.
SR9009 is a synthetic small molecule studied as an agonist of the REV-ERB nuclear receptors. It is not an approved medicine, and its effects in humans are not well characterized.